Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1843 | 5' | -54.5 | NC_001347.2 | + | 117146 | 0.66 | 0.977517 |
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Target: 5'- -gGCgGUUCgaCUGGaGGCGACGGg-- -3' miRNA: 3'- caUGgCGAGaaGACC-CCGCUGCUauc -5' |
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| 1843 | 5' | -54.5 | NC_001347.2 | + | 164584 | 0.66 | 0.972479 |
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Target: 5'- gGUGCUGCUgcgCgacacGGuGGCGGCGGUGGa -3' miRNA: 3'- -CAUGGCGAgaaGa----CC-CCGCUGCUAUC- -5' |
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| 1843 | 5' | -54.5 | NC_001347.2 | + | 94094 | 0.67 | 0.960049 |
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Target: 5'- cUGCCGCggaCUgcgCcggGGGGCGGCGGg-- -3' miRNA: 3'- cAUGGCGa--GAa--Ga--CCCCGCUGCUauc -5' |
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| 1843 | 5' | -54.5 | NC_001347.2 | + | 22271 | 0.71 | 0.836681 |
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Target: 5'- gGUACCGCUa--CUGuGGGCGAUGGg-- -3' miRNA: 3'- -CAUGGCGAgaaGAC-CCCGCUGCUauc -5' |
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| 1843 | 5' | -54.5 | NC_001347.2 | + | 43237 | 0.71 | 0.820126 |
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Target: 5'- -gACCGCgggCgcagUCcGGGGCGACGAc-- -3' miRNA: 3'- caUGGCGa--Ga---AGaCCCCGCUGCUauc -5' |
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| 1843 | 5' | -54.5 | NC_001347.2 | + | 191876 | 0.78 | 0.44553 |
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Target: 5'- cGUGCCGCUCgccgCUGGcGGCGACGu--- -3' miRNA: 3'- -CAUGGCGAGaa--GACC-CCGCUGCuauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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