Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18430 | 3' | -53.7 | NC_004681.1 | + | 70015 | 0.67 | 0.754222 |
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Target: 5'- -cGCUGGCUUUCGcGUCUcggAGCUCa -3' miRNA: 3'- uuCGGUCGGAAGCcCAGAuaaUCGAG- -5' |
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| 18430 | 3' | -53.7 | NC_004681.1 | + | 38897 | 0.67 | 0.733034 |
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Target: 5'- aAGGCCAGCCUgcuggcgUGGGUCaAUccaUGGC-Ca -3' miRNA: 3'- -UUCGGUCGGAa------GCCCAGaUA---AUCGaG- -5' |
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| 18430 | 3' | -53.7 | NC_004681.1 | + | 6411 | 0.68 | 0.707066 |
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Target: 5'- -cGUCGGCCUUCGGGUCauccgaccgcGGCa- -3' miRNA: 3'- uuCGGUCGGAAGCCCAGauaa------UCGag -5' |
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| 18430 | 3' | -53.7 | NC_004681.1 | + | 16946 | 0.68 | 0.689502 |
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Target: 5'- cGGCCAGCUUgugCcGGUCguugaugUAGCUCg -3' miRNA: 3'- uUCGGUCGGAa--GcCCAGaua----AUCGAG- -5' |
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| 18430 | 3' | -53.7 | NC_004681.1 | + | 56211 | 0.69 | 0.645058 |
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Target: 5'- uAGCCGGUCUcguUCGGGUCcucGUUGcGUUCg -3' miRNA: 3'- uUCGGUCGGA---AGCCCAGa--UAAU-CGAG- -5' |
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| 18430 | 3' | -53.7 | NC_004681.1 | + | 40283 | 0.69 | 0.633889 |
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Target: 5'- gGAGCCGGUCgauugcgCGGGUCUAU---CUCa -3' miRNA: 3'- -UUCGGUCGGaa-----GCCCAGAUAaucGAG- -5' |
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| 18430 | 3' | -53.7 | NC_004681.1 | + | 30135 | 0.72 | 0.491902 |
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Target: 5'- cAAGgCGGCCUgUGGGUCg---GGCUCa -3' miRNA: 3'- -UUCgGUCGGAaGCCCAGauaaUCGAG- -5' |
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| 18430 | 3' | -53.7 | NC_004681.1 | + | 28612 | 1.08 | 0.001814 |
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Target: 5'- uAAGCCAGCCUUCGGGUCUAUUAGCUCg -3' miRNA: 3'- -UUCGGUCGGAAGCCCAGAUAAUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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