Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18431 | 3' | -53.9 | NC_004681.1 | + | 18235 | 0.74 | 0.45709 |
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Target: 5'- gGCguggggAACGgGCCGACCUGACccGGUGCc -3' miRNA: 3'- -CGa-----UUGCgUGGCUGGACUGc-CCAUGu -5' |
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| 18431 | 3' | -53.9 | NC_004681.1 | + | 30196 | 0.74 | 0.437825 |
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Target: 5'- cCUGACgGCACCGACaaGAUGGaGUGCGg -3' miRNA: 3'- cGAUUG-CGUGGCUGgaCUGCC-CAUGU- -5' |
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| 18431 | 3' | -53.9 | NC_004681.1 | + | 19371 | 0.74 | 0.437825 |
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Target: 5'- --cGACcaCACCGGCCUgggcGACGGGUACAu -3' miRNA: 3'- cgaUUGc-GUGGCUGGA----CUGCCCAUGU- -5' |
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| 18431 | 3' | -53.9 | NC_004681.1 | + | 40217 | 0.74 | 0.419034 |
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Target: 5'- --gGGCGguCACCGACCUGGCgacccccgGGGUGCAg -3' miRNA: 3'- cgaUUGC--GUGGCUGGACUG--------CCCAUGU- -5' |
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| 18431 | 3' | -53.9 | NC_004681.1 | + | 15058 | 0.74 | 0.419034 |
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Target: 5'- uGCUcGCGCAgaUGACCcaGACGGGUGCGu -3' miRNA: 3'- -CGAuUGCGUg-GCUGGa-CUGCCCAUGU- -5' |
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| 18431 | 3' | -53.9 | NC_004681.1 | + | 16675 | 0.77 | 0.280025 |
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Target: 5'- uGCUGGCgacggcgcuccgaGCACCGGCCUGuuccCGGGUAUg -3' miRNA: 3'- -CGAUUG-------------CGUGGCUGGACu---GCCCAUGu -5' |
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| 18431 | 3' | -53.9 | NC_004681.1 | + | 42517 | 0.81 | 0.176456 |
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Target: 5'- gGCU-GCGCGCCGACgaGACGGGgGCGc -3' miRNA: 3'- -CGAuUGCGUGGCUGgaCUGCCCaUGU- -5' |
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| 18431 | 3' | -53.9 | NC_004681.1 | + | 29932 | 1.02 | 0.006399 |
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Target: 5'- aGCUAACGC-CCGACCUGACGGGUACAg -3' miRNA: 3'- -CGAUUGCGuGGCUGGACUGCCCAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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