Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18433 | 5' | -56.1 | NC_004681.1 | + | 70932 | 0.66 | 0.751983 |
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Target: 5'- -cGGCCGCUGAg-CGUGGaGACCgUGAc -3' miRNA: 3'- cuCUGGUGGCUagGCACCaCUGG-ACU- -5' |
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| 18433 | 5' | -56.1 | NC_004681.1 | + | 857 | 0.66 | 0.741894 |
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Target: 5'- aGGACC-UgGAUUCGUGGUucCCUGAa -3' miRNA: 3'- cUCUGGuGgCUAGGCACCAcuGGACU- -5' |
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| 18433 | 5' | -56.1 | NC_004681.1 | + | 33757 | 0.67 | 0.700597 |
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Target: 5'- gGAGguucGCCACCuGcUCCGUgaggaaGGUGACCUGc -3' miRNA: 3'- -CUC----UGGUGG-CuAGGCA------CCACUGGACu -5' |
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| 18433 | 5' | -56.1 | NC_004681.1 | + | 33467 | 0.67 | 0.679531 |
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Target: 5'- cGGGCCAgUGG-CCGUGGcUGAuCCUGGu -3' miRNA: 3'- cUCUGGUgGCUaGGCACC-ACU-GGACU- -5' |
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| 18433 | 5' | -56.1 | NC_004681.1 | + | 3326 | 0.72 | 0.442243 |
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Target: 5'- cGGACC-CCGAuUCCG-GGUGAgCUGGa -3' miRNA: 3'- cUCUGGuGGCU-AGGCaCCACUgGACU- -5' |
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| 18433 | 5' | -56.1 | NC_004681.1 | + | 54611 | 0.74 | 0.307186 |
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Target: 5'- cGGGCCACCGAUCC-UGG-GACCa-- -3' miRNA: 3'- cUCUGGUGGCUAGGcACCaCUGGacu -5' |
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| 18433 | 5' | -56.1 | NC_004681.1 | + | 7349 | 0.76 | 0.246213 |
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Target: 5'- uGGACgGCCGcgUCGUGGaUGGCCUGGa -3' miRNA: 3'- cUCUGgUGGCuaGGCACC-ACUGGACU- -5' |
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| 18433 | 5' | -56.1 | NC_004681.1 | + | 30276 | 1.09 | 0.001279 |
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Target: 5'- cGAGACCACCGAUCCGUGGUGACCUGAa -3' miRNA: 3'- -CUCUGGUGGCUAGGCACCACUGGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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