Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18435 | 3' | -55.7 | NC_004681.1 | + | 34650 | 0.66 | 0.769757 |
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Target: 5'- cGUUguuGUUUGCCcagUCCcUCAUGGCCUg -3' miRNA: 3'- -CAGuu-CGAGCGGa--AGGuAGUGCCGGG- -5' |
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| 18435 | 3' | -55.7 | NC_004681.1 | + | 41191 | 0.66 | 0.739485 |
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Target: 5'- uGUCAAGCUgCGCCUgcguaUCCAgucgauuacgUACGuGCuCCa -3' miRNA: 3'- -CAGUUCGA-GCGGA-----AGGUa---------GUGC-CG-GG- -5' |
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| 18435 | 3' | -55.7 | NC_004681.1 | + | 68901 | 0.66 | 0.759788 |
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Target: 5'- aUCGAGg-CGCUgaCCAucgUCACGGCCg -3' miRNA: 3'- cAGUUCgaGCGGaaGGU---AGUGCCGGg -5' |
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| 18435 | 3' | -55.7 | NC_004681.1 | + | 71634 | 0.66 | 0.739485 |
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Target: 5'- -aCcGGCUCagaGCCUUCC-UCGCGGUaguCCa -3' miRNA: 3'- caGuUCGAG---CGGAAGGuAGUGCCG---GG- -5' |
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| 18435 | 3' | -55.7 | NC_004681.1 | + | 17357 | 0.67 | 0.708281 |
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Target: 5'- cUCAAGguCUCGCCgacugcUCCAgCGCGGUCg -3' miRNA: 3'- cAGUUC--GAGCGGa-----AGGUaGUGCCGGg -5' |
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| 18435 | 3' | -55.7 | NC_004681.1 | + | 22573 | 0.67 | 0.687102 |
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Target: 5'- gGUCuGGgUCGCCagCCGcaUCaACGGCCUg -3' miRNA: 3'- -CAGuUCgAGCGGaaGGU--AG-UGCCGGG- -5' |
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| 18435 | 3' | -55.7 | NC_004681.1 | + | 54998 | 0.68 | 0.676432 |
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Target: 5'- -gCAAGCUCggccugaaguGCCUUCagGUCGC-GCCCg -3' miRNA: 3'- caGUUCGAG----------CGGAAGg-UAGUGcCGGG- -5' |
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| 18435 | 3' | -55.7 | NC_004681.1 | + | 64679 | 0.68 | 0.64422 |
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Target: 5'- -cCAAGCUgGgCCUgacauaCAUCGCaGGCCCc -3' miRNA: 3'- caGUUCGAgC-GGAag----GUAGUG-CCGGG- -5' |
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| 18435 | 3' | -55.7 | NC_004681.1 | + | 30676 | 1.11 | 0.000915 |
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Target: 5'- gGUCAAGCUCGCCUUCCAUCACGGCCCu -3' miRNA: 3'- -CAGUUCGAGCGGAAGGUAGUGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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