Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18436 | 5' | -52.5 | NC_004681.1 | + | 56423 | 0.66 | 0.907399 |
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Target: 5'- gCACCuUGGACGccuuggcgccguGGGUGUGcUUGACc -3' miRNA: 3'- aGUGG-ACCUGCu-----------CCCACAUuAGCUGu -5' |
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| 18436 | 5' | -52.5 | NC_004681.1 | + | 42418 | 0.66 | 0.902073 |
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Target: 5'- gCGCgUGGGCGGGGaugGUGAgcUUGACGa -3' miRNA: 3'- aGUGgACCUGCUCCca-CAUU--AGCUGU- -5' |
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| 18436 | 5' | -52.5 | NC_004681.1 | + | 64749 | 0.67 | 0.865014 |
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Target: 5'- aUCGuCCgguauGGCGAucuGGGUGUAGUCGAUg -3' miRNA: 3'- -AGU-GGac---CUGCU---CCCACAUUAGCUGu -5' |
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| 18436 | 5' | -52.5 | NC_004681.1 | + | 32937 | 0.68 | 0.856859 |
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Target: 5'- aUCGCCUGaaggacuucaucGGCGGcGGGUGcu-UCGGCAa -3' miRNA: 3'- -AGUGGAC------------CUGCU-CCCACauuAGCUGU- -5' |
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| 18436 | 5' | -52.5 | NC_004681.1 | + | 22543 | 0.68 | 0.821999 |
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Target: 5'- cUCACCcgcgUGGACGuagaacuucAGGGUGUAGUgCGGa- -3' miRNA: 3'- -AGUGG----ACCUGC---------UCCCACAUUA-GCUgu -5' |
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| 18436 | 5' | -52.5 | NC_004681.1 | + | 7506 | 0.69 | 0.764014 |
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Target: 5'- -uGCCUGGGCcuGGGUGcGcgCGACGu -3' miRNA: 3'- agUGGACCUGcuCCCACaUuaGCUGU- -5' |
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| 18436 | 5' | -52.5 | NC_004681.1 | + | 43811 | 0.7 | 0.711923 |
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Target: 5'- uUCACCgaGGGCGGcGGUGUAcugCGGCGg -3' miRNA: 3'- -AGUGGa-CCUGCUcCCACAUua-GCUGU- -5' |
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| 18436 | 5' | -52.5 | NC_004681.1 | + | 65202 | 0.72 | 0.603253 |
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Target: 5'- aCGCCUGGugGgAGGGUcaGgauAUCGACc -3' miRNA: 3'- aGUGGACCugC-UCCCA--Cau-UAGCUGu -5' |
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| 18436 | 5' | -52.5 | NC_004681.1 | + | 31610 | 1.09 | 0.002887 |
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Target: 5'- gUCACCUGGACGAGGGUGUAAUCGACAc -3' miRNA: 3'- -AGUGGACCUGCUCCCACAUUAGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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