miRNA: 3'- uCCUUC-CGCAGGAGCuCU----UCgACCUG- -5'
miRNA: 3'- uCCUUCCG--CAGG---------AGCUCUUCGACCUg -5'
miRNA: 3'- -UCCUUCCGCAGgaGcUCuuCGACCUG- -5'
miRNA: 3'- -UCCUUCCGCaGGA---GCUCUuCGACCUG- -5'
miRNA: 3'- -UCCUUCCGCAGGAGCUCUUCGACCUG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18437 | 3' | -56.2 | NC_004681.1 | + | 40856 | 0.73 | 0.349016 |
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Target: 5'- cGGAGGaGCGUCCUCGcGGuacaAGgUGGGCu -3' miRNA: 3'- uCCUUC-CGCAGGAGCuCU----UCgACCUG- -5' |
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| 18437 | 3' | -56.2 | NC_004681.1 | + | 51495 | 0.74 | 0.31738 |
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Target: 5'- cGGAGGGCacGUUCugcugcaugUCGAGGAGCUGGGg -3' miRNA: 3'- uCCUUCCG--CAGG---------AGCUCUUCGACCUg -5' |
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| 18437 | 3' | -56.2 | NC_004681.1 | + | 10004 | 0.75 | 0.274062 |
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Target: 5'- gGGGAAGGCGUCggCcAGcuGCUGGGCg -3' miRNA: 3'- -UCCUUCCGCAGgaGcUCuuCGACCUG- -5' |
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| 18437 | 3' | -56.2 | NC_004681.1 | + | 475 | 0.82 | 0.091901 |
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Target: 5'- uGGGGAGGCG-CCUggcCGAGAuGCUGGGCg -3' miRNA: 3'- -UCCUUCCGCaGGA---GCUCUuCGACCUG- -5' |
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| 18437 | 3' | -56.2 | NC_004681.1 | + | 32064 | 1.08 | 0.001227 |
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Target: 5'- aAGGAAGGCGUCCUCGAGAAGCUGGACc -3' miRNA: 3'- -UCCUUCCGCAGGAGCUCUUCGACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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