miRNA: 3'- -UCCU--UCC-GCaGGAGCUcuuCGACCUG- -5'
miRNA: 3'- -UCCUUCCGCa---GGAgCUCUUc---GACCUg -5'
miRNA: 3'- uCCUUCCGCaGGaGcUCUUCGa--CCUG- -5'
miRNA: 3'- -UCCuuCCGCAGGAgCUCU---UCGA---CCUG- -5'
miRNA: 3'- -UCCUUCCGCaGGA---GCUCUuCGACCUG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18437 | 3' | -56.2 | NC_004681.1 | + | 5470 | 0.69 | 0.558507 |
|
Target: 5'- uGGGAguGGGuCG-CCUCGAc--GCUGGACa -3' miRNA: 3'- -UCCU--UCC-GCaGGAGCUcuuCGACCUG- -5' |
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| 18437 | 3' | -56.2 | NC_004681.1 | + | 1866 | 0.67 | 0.65498 |
|
Target: 5'- gAGGAGGGCGauggCCUgGGGAAcuacCUGGGu -3' miRNA: 3'- -UCCUUCCGCa---GGAgCUCUUc---GACCUg -5' |
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| 18437 | 3' | -56.2 | NC_004681.1 | + | 1281 | 0.67 | 0.665721 |
|
Target: 5'- cGGAGGGCG-CC-CaGGAAGUcugGGACg -3' miRNA: 3'- uCCUUCCGCaGGaGcUCUUCGa--CCUG- -5' |
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| 18437 | 3' | -56.2 | NC_004681.1 | + | 1029 | 0.69 | 0.569105 |
|
Target: 5'- gAGGucGGCGUCUUgGAGGaugAGCUcgaGGAUg -3' miRNA: 3'- -UCCuuCCGCAGGAgCUCU---UCGA---CCUG- -5' |
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| 18437 | 3' | -56.2 | NC_004681.1 | + | 475 | 0.82 | 0.091901 |
|
Target: 5'- uGGGGAGGCG-CCUggcCGAGAuGCUGGGCg -3' miRNA: 3'- -UCCUUCCGCaGGA---GCUCUuCGACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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