Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18439 | 3' | -57.4 | NC_004681.1 | + | 18326 | 0.67 | 0.640424 |
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Target: 5'- cGGUUCGUCCGGuGGCGGUG-GcGGGc -3' miRNA: 3'- cUCAGGCAGGUC-CUGCCGCaCuUCCu -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 38810 | 0.67 | 0.608707 |
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Target: 5'- aAGUCCGccgagaccaUCCGGGccuacgGCGGCagcGUGGAGGGc -3' miRNA: 3'- cUCAGGC---------AGGUCC------UGCCG---CACUUCCU- -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 2014 | 0.68 | 0.598161 |
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Target: 5'- --aUCCGgccgcggCCAGGugGuCGUGGAGGGc -3' miRNA: 3'- cucAGGCa------GGUCCugCcGCACUUCCU- -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 10528 | 0.68 | 0.598161 |
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Target: 5'- aAGUCCGaCCGcGGAaucccgcaGGCGgUGGAGGAa -3' miRNA: 3'- cUCAGGCaGGU-CCUg-------CCGC-ACUUCCU- -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 34338 | 0.68 | 0.55632 |
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Target: 5'- aAGUCUuacgCCGcGGCGGCGUGAGGGu -3' miRNA: 3'- cUCAGGca--GGUcCUGCCGCACUUCCu -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 67674 | 0.69 | 0.505313 |
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Target: 5'- uGGUCaCuUUCAGGA-GGCGUGGAGGGc -3' miRNA: 3'- cUCAG-GcAGGUCCUgCCGCACUUCCU- -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 48752 | 0.7 | 0.485468 |
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Target: 5'- uGGUCCagcaccUCCAGGACGGCGgcuacaccUGGAGuGAg -3' miRNA: 3'- cUCAGGc-----AGGUCCUGCCGC--------ACUUC-CU- -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 42296 | 0.7 | 0.446948 |
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Target: 5'- --aUCCGcUCCAGuuCGGCGUGAcGGAg -3' miRNA: 3'- cucAGGC-AGGUCcuGCCGCACUuCCU- -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 27047 | 0.71 | 0.410189 |
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Target: 5'- cGAGUCCGU---GGuCGGCGUcGAGGGAc -3' miRNA: 3'- -CUCAGGCAgguCCuGCCGCA-CUUCCU- -5' |
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| 18439 | 3' | -57.4 | NC_004681.1 | + | 32798 | 1.08 | 0.00109 |
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Target: 5'- gGAGUCCGUCCAGGACGGCGUGAAGGAg -3' miRNA: 3'- -CUCAGGCAGGUCCUGCCGCACUUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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