Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18439 | 5' | -56.1 | NC_004681.1 | + | 58030 | 0.66 | 0.761908 |
Target: 5'- gGCUCAagcagacggaggaaUCGGCGgCAUGagcaacgagaaCUGGCGCGUg -3' miRNA: 3'- -CGAGU--------------AGUUGCgGUGCg----------GACCGUGCA- -5' |
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18439 | 5' | -56.1 | NC_004681.1 | + | 52722 | 0.66 | 0.764897 |
Target: 5'- aGCUCAugUCGAuugcgguuuCGCUAcCGCCUGGCuGCa- -3' miRNA: 3'- -CGAGU--AGUU---------GCGGU-GCGGACCG-UGca -5' |
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18439 | 5' | -56.1 | NC_004681.1 | + | 48574 | 0.66 | 0.764897 |
Target: 5'- uGCUgCAUCGugGgCCACGCCcucgcagaGGCAgGc -3' miRNA: 3'- -CGA-GUAGUugC-GGUGCGGa-------CCGUgCa -5' |
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18439 | 5' | -56.1 | NC_004681.1 | + | 68247 | 0.66 | 0.768866 |
Target: 5'- uCUCAUCGacuACGCCguGCGacguuucgcaccaacCCUGGCAgGUa -3' miRNA: 3'- cGAGUAGU---UGCGG--UGC---------------GGACCGUgCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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