miRNA: 3'- cGAGua-GUUGCGGuGCGGAC--CGUGCa -5'
miRNA: 3'- cGAGUAGuuGCGGUGCGG---ACCG-UGCa -5'
miRNA: 3'- -CGAGUa-------GUUGCGGUGcGGACCGUGca -5'
miRNA: 3'- cGAGUAGU---UGCGG--UGC---------------GGACCGUgCA- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18439 | 5' | -56.1 | NC_004681.1 | + | 64912 | 0.69 | 0.543302 |
|
Target: 5'- uCUCccgCGGCGCC-CGCCUGgaGCACGa -3' miRNA: 3'- cGAGua-GUUGCGGuGCGGAC--CGUGCa -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 65721 | 0.7 | 0.532877 |
|
Target: 5'- cCUUGUCcuCGCCACGCCacuUGGCgGCGa -3' miRNA: 3'- cGAGUAGuuGCGGUGCGG---ACCG-UGCa -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 66296 | 0.68 | 0.639228 |
|
Target: 5'- aGUUCGgguccuugCAGCGCCACaUCUGGUACa- -3' miRNA: 3'- -CGAGUa-------GUUGCGGUGcGGACCGUGca -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 68247 | 0.66 | 0.768866 |
|
Target: 5'- uCUCAUCGacuACGCCguGCGacguuucgcaccaacCCUGGCAgGUa -3' miRNA: 3'- cGAGUAGU---UGCGG--UGC---------------GGACCGUgCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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