miRNA: 3'- -CGAG--UAGUUGCGGugC-GGA-CCGUGCa -5'
miRNA: 3'- -CGAGUAGU-UgCGgUGCG-GA-CCGUGca -5'
miRNA: 3'- cGAGUaGUUGCGGUG---CGgACCGUGca -5'
miRNA: 3'- -CGA-GU-----AgUUGCGGUGCGG---ACCGUGca -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18439 | 5' | -56.1 | NC_004681.1 | + | 7494 | 0.72 | 0.424153 |
|
Target: 5'- cGCUCcgGUCGAUGCCugGgCCUgGGUGCGc -3' miRNA: 3'- -CGAG--UAGUUGCGGugC-GGA-CCGUGCa -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 7382 | 0.66 | 0.724184 |
|
Target: 5'- aGCUCGUCGcAgGCgGCGCaCUcGGCGCc- -3' miRNA: 3'- -CGAGUAGU-UgCGgUGCG-GA-CCGUGca -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 6471 | 0.68 | 0.639228 |
|
Target: 5'- cCUCGaCAGCGCCAUcggGCgUGGCGCc- -3' miRNA: 3'- cGAGUaGUUGCGGUG---CGgACCGUGca -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 6355 | 0.7 | 0.522524 |
|
Target: 5'- cGCUgCAgcccuUgGGCGCCACGCCcgaUGGCGCu- -3' miRNA: 3'- -CGA-GU-----AgUUGCGGUGCGG---ACCGUGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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