miRNA: 3'- cGAGuaGUUGCGgUGCGgACC-GUGCa -5'
miRNA: 3'- -CGAG------UAGUUGCGG------------UGCGGACCGU-GCa -5'
miRNA: 3'- -CGAGUAGUUGcGGUGCGGA-CCGUgca -5'
miRNA: 3'- -CGAGU--AGUU---------GCGGU-GCGGACCG-UGca -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18439 | 5' | -56.1 | NC_004681.1 | + | 57895 | 0.7 | 0.522524 |
|
Target: 5'- cCUCggCGGCGCgGCGCgUGGuCGCGg -3' miRNA: 3'- cGAGuaGUUGCGgUGCGgACC-GUGCa -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 51096 | 0.71 | 0.442968 |
|
Target: 5'- aGCUCcccgacGUCGACGCCgguguuccauugACGCUUGGCGuCGg -3' miRNA: 3'- -CGAG------UAGUUGCGG------------UGCGGACCGU-GCa -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 14417 | 0.72 | 0.405825 |
|
Target: 5'- aGCUCAUUGGC-CCGCGCCUcGGCGa-- -3' miRNA: 3'- -CGAGUAGUUGcGGUGCGGA-CCGUgca -5' |
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| 18439 | 5' | -56.1 | NC_004681.1 | + | 52722 | 0.66 | 0.764897 |
|
Target: 5'- aGCUCAugUCGAuugcgguuuCGCUAcCGCCUGGCuGCa- -3' miRNA: 3'- -CGAGU--AGUU---------GCGGU-GCGGACCG-UGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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