Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
18443 | 5' | -55.4 | NC_004681.1 | + | 35296 | 1.1 | 0.001214 |
Target: 5'- gGUGAGGGGCGAAUCCAUGUCGGGAUCg -3' miRNA: 3'- -CACUCCCCGCUUAGGUACAGCCCUAG- -5' |
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18443 | 5' | -55.4 | NC_004681.1 | + | 65330 | 0.71 | 0.498836 |
Target: 5'- -cGAGGuGCGGcaggCCGcUGUCGGGGUCg -3' miRNA: 3'- caCUCCcCGCUua--GGU-ACAGCCCUAG- -5' |
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18443 | 5' | -55.4 | NC_004681.1 | + | 1613 | 0.69 | 0.614906 |
Target: 5'- --cAGGGGUGAGUCCGaagCGGGAc- -3' miRNA: 3'- cacUCCCCGCUUAGGUacaGCCCUag -5' |
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18443 | 5' | -55.4 | NC_004681.1 | + | 19838 | 0.67 | 0.708313 |
Target: 5'- -aGcGGGGCGAucagcaacggccugAUCCAUGUguGGAUCa -3' miRNA: 3'- caCuCCCCGCU--------------UAGGUACAgcCCUAG- -5' |
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18443 | 5' | -55.4 | NC_004681.1 | + | 2606 | 0.67 | 0.742687 |
Target: 5'- -aGAuGGGUGAcAUCCAaGUCGGGGa- -3' miRNA: 3'- caCUcCCCGCU-UAGGUaCAGCCCUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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