Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18444 | 5' | -53.4 | NC_004681.1 | + | 17942 | 0.66 | 0.880342 |
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Target: 5'- aGGUGAGUg--CCGAGGGCg------ -3' miRNA: 3'- cCCACUCAggaGGCUCCCGaaauagu -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 51751 | 0.67 | 0.848328 |
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Target: 5'- gGGGUGGGgaugaCgUCgGAGGGCUg-GUCu -3' miRNA: 3'- -CCCACUCa----GgAGgCUCCCGAaaUAGu -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 25201 | 0.68 | 0.803396 |
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Target: 5'- gGGGUGGGUCCgcggccaaCCGAGGuGCc------ -3' miRNA: 3'- -CCCACUCAGGa-------GGCUCC-CGaaauagu -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 75027 | 0.69 | 0.743819 |
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Target: 5'- -aGUGAGggCUCCGuGGGCUccgUGUCGu -3' miRNA: 3'- ccCACUCagGAGGCuCCCGAa--AUAGU- -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 53572 | 0.69 | 0.718739 |
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Target: 5'- aGGGUGcggcGUUCgaugucuucguugCCGAGGGCUUcgAUCAg -3' miRNA: 3'- -CCCACu---CAGGa------------GGCUCCCGAAa-UAGU- -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 7736 | 0.7 | 0.701742 |
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Target: 5'- aGGUGgaGGUCC-CCGAGGGCg------ -3' miRNA: 3'- cCCAC--UCAGGaGGCUCCCGaaauagu -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 46708 | 0.7 | 0.669448 |
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Target: 5'- cGGGgcgGGGUCCUCggCGAgugggccggucuGGGCUUcGUCAg -3' miRNA: 3'- -CCCa--CUCAGGAG--GCU------------CCCGAAaUAGU- -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 75110 | 0.71 | 0.636844 |
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Target: 5'- uGGGUGAGU-CUCUGuAGGGCUa----- -3' miRNA: 3'- -CCCACUCAgGAGGC-UCCCGAaauagu -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 70419 | 0.72 | 0.561042 |
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Target: 5'- uGGGUGAGUCUgucaGAGGGCUa----- -3' miRNA: 3'- -CCCACUCAGGagg-CUCCCGAaauagu -5' |
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| 18444 | 5' | -53.4 | NC_004681.1 | + | 35432 | 1.12 | 0.001548 |
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Target: 5'- cGGGUGAGUCCUCCGAGGGCUUUAUCAa -3' miRNA: 3'- -CCCACUCAGGAGGCUCCCGAAAUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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