Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18445 | 5' | -56.5 | NC_004681.1 | + | 39955 | 0.68 | 0.636968 |
Target: 5'- aGUCGAuCCgCgCGCGUCgGCGCCGg--- -3' miRNA: 3'- -CAGCUuGG-G-GUGCAGaCGCGGCaaca -5' |
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18445 | 5' | -56.5 | NC_004681.1 | + | 35538 | 1.07 | 0.001523 |
Target: 5'- uGUCGAACCCCACGUCUGCGCCGUUGUa -3' miRNA: 3'- -CAGCUUGGGGUGCAGACGCGGCAACA- -5' |
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18445 | 5' | -56.5 | NC_004681.1 | + | 15662 | 0.66 | 0.761958 |
Target: 5'- gGUC-AugCCCugGUCgGUGCCGgUGa -3' miRNA: 3'- -CAGcUugGGGugCAGaCGCGGCaACa -5' |
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18445 | 5' | -56.5 | NC_004681.1 | + | 6345 | 0.68 | 0.626294 |
Target: 5'- -gCGAACUCCGCG-CUGCaGCCcUUGg -3' miRNA: 3'- caGCUUGGGGUGCaGACG-CGGcAACa -5' |
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18445 | 5' | -56.5 | NC_004681.1 | + | 2282 | 0.67 | 0.679531 |
Target: 5'- uGUCGucUCCCGCGcaCUGCGCCGc--- -3' miRNA: 3'- -CAGCuuGGGGUGCa-GACGCGGCaaca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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