Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18447 | 3' | -59.1 | NC_004681.1 | + | 3342 | 0.71 | 0.320186 |
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Target: 5'- aGCgAGCCaggaagaGCgCAGCCUCGGCCAggucCCGGUu -3' miRNA: 3'- -CG-UCGG-------CGaGUUGGAGCCGGU----GGUCA- -5' |
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| 18447 | 3' | -59.1 | NC_004681.1 | + | 57681 | 0.71 | 0.313455 |
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Target: 5'- cGCAG-CGCUCGAcgcaauCCUCGGCCAacccgaCAGg -3' miRNA: 3'- -CGUCgGCGAGUU------GGAGCCGGUg-----GUCa -5' |
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| 18447 | 3' | -59.1 | NC_004681.1 | + | 27915 | 0.72 | 0.284841 |
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Target: 5'- aGCGGCaCGCggGGCCUCGGCgGCaCGGc -3' miRNA: 3'- -CGUCG-GCGagUUGGAGCCGgUG-GUCa -5' |
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| 18447 | 3' | -59.1 | NC_004681.1 | + | 46450 | 0.72 | 0.252026 |
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Target: 5'- cGCGGCCuGCUCcgguACCUCGGCCuggaGGUc -3' miRNA: 3'- -CGUCGG-CGAGu---UGGAGCCGGugg-UCA- -5' |
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| 18447 | 3' | -59.1 | NC_004681.1 | + | 9925 | 0.73 | 0.236226 |
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Target: 5'- uGCGGCCGCUgcggaCAACCUUGGUCucgaccucguccucgACCAGc -3' miRNA: 3'- -CGUCGGCGA-----GUUGGAGCCGG---------------UGGUCa -5' |
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| 18447 | 3' | -59.1 | NC_004681.1 | + | 55889 | 0.73 | 0.233871 |
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Target: 5'- aGCAGCCGCUCucGACCUauggcgaGGUCGuuguuCCAGUa -3' miRNA: 3'- -CGUCGGCGAG--UUGGAg------CCGGU-----GGUCA- -5' |
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| 18447 | 3' | -59.1 | NC_004681.1 | + | 8985 | 0.73 | 0.233871 |
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Target: 5'- --cGCUGUUCGgcgccACCUCGGCCACCGu- -3' miRNA: 3'- cguCGGCGAGU-----UGGAGCCGGUGGUca -5' |
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| 18447 | 3' | -59.1 | NC_004681.1 | + | 65783 | 0.75 | 0.167099 |
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Target: 5'- gGCGGaguucguCCGuCUCGGCCUCGGCCGCCc-- -3' miRNA: 3'- -CGUC-------GGC-GAGUUGGAGCCGGUGGuca -5' |
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| 18447 | 3' | -59.1 | NC_004681.1 | + | 36196 | 1.08 | 0.000638 |
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Target: 5'- cGCAGCCGCUCAACCUCGGCCACCAGUu -3' miRNA: 3'- -CGUCGGCGAGUUGGAGCCGGUGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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