Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 18449 | 3' | -54.8 | NC_004681.1 | + | 45297 | 0.76 | 0.302843 |
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Target: 5'- uCAUCCCCGGugaUUCUCGAUGGCGCg- -3' miRNA: 3'- cGUGGGGGCUg--AAGAGCUACUGUGgg -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 33385 | 0.77 | 0.261532 |
|
Target: 5'- gGCGCgaCCGGCg--UCGAUGACACCCg -3' miRNA: 3'- -CGUGggGGCUGaagAGCUACUGUGGG- -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 1267 | 0.79 | 0.197826 |
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Target: 5'- -gGCCCCCGACaUgUCGGagGGCGCCCa -3' miRNA: 3'- cgUGGGGGCUGaAgAGCUa-CUGUGGG- -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 62665 | 0.8 | 0.182969 |
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Target: 5'- cGCGCUCCCGGCguuccaUCUCcugGAUGACACCg -3' miRNA: 3'- -CGUGGGGGCUGa-----AGAG---CUACUGUGGg -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 20164 | 0.86 | 0.079006 |
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Target: 5'- cGCugCCCCGGCUgcgCUCGGUGAucgacuuguCGCCCa -3' miRNA: 3'- -CGugGGGGCUGAa--GAGCUACU---------GUGGG- -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 37517 | 1.14 | 0.000922 |
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Target: 5'- aGCACCCCCGACUUCUCGAUGACACCCu -3' miRNA: 3'- -CGUGGGGGCUGAAGAGCUACUGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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