Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18449 | 3' | -54.8 | NC_004681.1 | + | 2300 | 0.67 | 0.803618 |
Target: 5'- aCACCCUCGAUgacaUCGAauGCACCg -3' miRNA: 3'- cGUGGGGGCUGaag-AGCUacUGUGGg -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 1366 | 0.68 | 0.765327 |
Target: 5'- gGCGCCCuCCGACaUgUCGggGGCcacgaguuCCCc -3' miRNA: 3'- -CGUGGG-GGCUGaAgAGCuaCUGu-------GGG- -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 1267 | 0.79 | 0.197826 |
Target: 5'- -gGCCCCCGACaUgUCGGagGGCGCCCa -3' miRNA: 3'- cgUGGGGGCUGaAgAGCUa-CUGUGGG- -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 709 | 0.72 | 0.514771 |
Target: 5'- gGCGCggaggaaguUCUCGACggCgUCGAUGGCGCCCu -3' miRNA: 3'- -CGUG---------GGGGCUGaaG-AGCUACUGUGGG- -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 629 | 0.66 | 0.863811 |
Target: 5'- cGCGCCUUCGGCccggUGAUcGACGCCg -3' miRNA: 3'- -CGUGGGGGCUGaagaGCUA-CUGUGGg -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 561 | 0.69 | 0.704249 |
Target: 5'- gGCGCCuCCCcACgaaCUCGGccagguugGACGCCCa -3' miRNA: 3'- -CGUGG-GGGcUGaa-GAGCUa-------CUGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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