Results 61 - 66 of 66 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18449 | 3' | -54.8 | NC_004681.1 | + | 13378 | 0.7 | 0.619467 |
Target: 5'- gGCGCCCUCGGa--CUCGAUGGC-CUg -3' miRNA: 3'- -CGUGGGGGCUgaaGAGCUACUGuGGg -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 16054 | 0.7 | 0.647176 |
Target: 5'- gGCACaCCCCGAaugaacgaGAUGGCGCCa -3' miRNA: 3'- -CGUG-GGGGCUgaagag--CUACUGUGGg -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 58878 | 0.7 | 0.651435 |
Target: 5'- cGCGCgCCUCGACggugCGcGUGACACCa -3' miRNA: 3'- -CGUG-GGGGCUGaagaGC-UACUGUGGg -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 561 | 0.69 | 0.704249 |
Target: 5'- gGCGCCuCCCcACgaaCUCGGccagguugGACGCCCa -3' miRNA: 3'- -CGUGG-GGGcUGaa-GAGCUa-------CUGUGGG- -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 5686 | 0.68 | 0.714655 |
Target: 5'- aGCGgCCaguuCGACUguugcgCUUGAUGGCgACCCg -3' miRNA: 3'- -CGUgGGg---GCUGAa-----GAGCUACUG-UGGG- -5' |
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18449 | 3' | -54.8 | NC_004681.1 | + | 62665 | 0.8 | 0.182969 |
Target: 5'- cGCGCUCCCGGCguuccaUCUCcugGAUGACACCg -3' miRNA: 3'- -CGUGGGGGCUGa-----AGAG---CUACUGUGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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