Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18449 | 3' | -54.8 | NC_004681.1 | + | 35389 | 0.67 | 0.78477 |
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Target: 5'- cCACCgguggCCgGAUUcCUCGAcgGGCACCCa -3' miRNA: 3'- cGUGG-----GGgCUGAaGAGCUa-CUGUGGG- -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 54585 | 0.68 | 0.762364 |
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Target: 5'- aGCugCCCCccgcaaucaucgugGACa--UCGAcGGCACCCu -3' miRNA: 3'- -CGugGGGG--------------CUGaagAGCUaCUGUGGG- -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 16473 | 0.68 | 0.755407 |
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Target: 5'- aGC-CCCCCacgGACUaCUCGgcGccGCGCCCc -3' miRNA: 3'- -CGuGGGGG---CUGAaGAGCuaC--UGUGGG- -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 7324 | 0.68 | 0.724983 |
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Target: 5'- cCACCCCCGGCacC-CGGggcUGAC-CCCg -3' miRNA: 3'- cGUGGGGGCUGaaGaGCU---ACUGuGGG- -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 44739 | 0.69 | 0.704249 |
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Target: 5'- gGCGCCUUCGACg--UCGAgGACuCCCu -3' miRNA: 3'- -CGUGGGGGCUGaagAGCUaCUGuGGG- -5' |
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| 18449 | 3' | -54.8 | NC_004681.1 | + | 37517 | 1.14 | 0.000922 |
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Target: 5'- aGCACCCCCGACUUCUCGAUGACACCCu -3' miRNA: 3'- -CGUGGGGGCUGAAGAGCUACUGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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