miRNA: 3'- cUCUaCGAGCGGGAG------CugCAGCuCCU- -5'
miRNA: 3'- cUCUACGAGC--GGGAGCugCaGCUCCU- -5'
miRNA: 3'- -CUCUACG--AGCGGGA----------GCUGCaGCUCCU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18451 | 5' | -57.2 | NC_004681.1 | + | 10847 | 0.66 | 0.710157 |
|
Target: 5'- uGGA-GCUUGCCCUUgucccaGugGUCGcGGAc -3' miRNA: 3'- cUCUaCGAGCGGGAG------CugCAGCuCCU- -5' |
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| 18451 | 5' | -57.2 | NC_004681.1 | + | 6998 | 0.76 | 0.213321 |
|
Target: 5'- aGGAUGUUCGgaCCCUCGugGaCGAGGGg -3' miRNA: 3'- cUCUACGAGC--GGGAGCugCaGCUCCU- -5' |
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| 18451 | 5' | -57.2 | NC_004681.1 | + | 1900 | 0.67 | 0.647226 |
|
Target: 5'- uGGGAUGUacUCGCUCUgccuggccuaCGACGgUGAGGAg -3' miRNA: 3'- -CUCUACG--AGCGGGA----------GCUGCaGCUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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