Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18454 | 3' | -59.6 | NC_004681.1 | + | 39567 | 0.69 | 0.331388 |
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Target: 5'- cUUGAGCCAGCGCacCGCggaGGUgUGUCGc -3' miRNA: 3'- -AACUUGGUCGCG--GCGaa-CCGgACGGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 9100 | 0.69 | 0.315943 |
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Target: 5'- ---cACCGGCGCCGCUggcucgGGCuCUGgCGu -3' miRNA: 3'- aacuUGGUCGCGGCGAa-----CCG-GACgGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 32326 | 0.7 | 0.308427 |
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Target: 5'- -cGAgGCCGcGCuGCCGCUgcgaUGGCCUGCUc -3' miRNA: 3'- aaCU-UGGU-CG-CGGCGA----ACCGGACGGu -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 52248 | 0.71 | 0.240725 |
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Target: 5'- -aGGACCAccaccauguGCaGCUGCUUGGCC-GCCAc -3' miRNA: 3'- aaCUUGGU---------CG-CGGCGAACCGGaCGGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 18482 | 0.74 | 0.150243 |
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Target: 5'- ---cGCCgcGGcCGCCGCcUGGCCUGCCAu -3' miRNA: 3'- aacuUGG--UC-GCGGCGaACCGGACGGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 6280 | 0.76 | 0.11766 |
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Target: 5'- -cGAACgAGgGCCGCgagaucaugGGCCUGCCGc -3' miRNA: 3'- aaCUUGgUCgCGGCGaa-------CCGGACGGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 25287 | 0.77 | 0.09176 |
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Target: 5'- ---uGCCAGCGCCGCcaggGGCCUGaCCGg -3' miRNA: 3'- aacuUGGUCGCGGCGaa--CCGGAC-GGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 40000 | 1.06 | 0.000692 |
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Target: 5'- cUUGAACCAGCGCCGCUUGGCCUGCCAg -3' miRNA: 3'- -AACUUGGUCGCGGCGAACCGGACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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