Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18454 | 3' | -59.6 | NC_004681.1 | + | 18482 | 0.74 | 0.150243 |
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Target: 5'- ---cGCCgcGGcCGCCGCcUGGCCUGCCAu -3' miRNA: 3'- aacuUGG--UC-GCGGCGaACCGGACGGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 52248 | 0.71 | 0.240725 |
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Target: 5'- -aGGACCAccaccauguGCaGCUGCUUGGCC-GCCAc -3' miRNA: 3'- aaCUUGGU---------CG-CGGCGAACCGGaCGGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 32326 | 0.7 | 0.308427 |
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Target: 5'- -cGAgGCCGcGCuGCCGCUgcgaUGGCCUGCUc -3' miRNA: 3'- aaCU-UGGU-CG-CGGCGA----ACCGGACGGu -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 39567 | 0.69 | 0.331388 |
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Target: 5'- cUUGAGCCAGCGCacCGCggaGGUgUGUCGc -3' miRNA: 3'- -AACUUGGUCGCG--GCGaa-CCGgACGGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 49696 | 0.69 | 0.355583 |
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Target: 5'- -gGAAUCuGCGCCuuCUUGGCCcGCCu -3' miRNA: 3'- aaCUUGGuCGCGGc-GAACCGGaCGGu -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 28129 | 0.68 | 0.38973 |
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Target: 5'- -aGcGCCAGCGCCGUc-GGUcuugCUGCCAc -3' miRNA: 3'- aaCuUGGUCGCGGCGaaCCG----GACGGU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 74908 | 0.67 | 0.434377 |
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Target: 5'- -aGGACCAGUGCuuccgcgagcuguCGCUUGaGCUUGCgGa -3' miRNA: 3'- aaCUUGGUCGCG-------------GCGAAC-CGGACGgU- -5' |
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| 18454 | 3' | -59.6 | NC_004681.1 | + | 49672 | 0.66 | 0.493856 |
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Target: 5'- cUGAugCAGuCGuuGgUgccucgGGCCUGCCc -3' miRNA: 3'- aACUugGUC-GCggCgAa-----CCGGACGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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