Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18454 | 5' | -59 | NC_004681.1 | + | 1602 | 0.67 | 0.520366 |
Target: 5'- aGCGUCuccuuGGGUGAuucccGCCcAUCCUGCAa -3' miRNA: 3'- cUGCAGu----CCCGCUu----CGGcUAGGGCGU- -5' |
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18454 | 5' | -59 | NC_004681.1 | + | 1038 | 0.66 | 0.57158 |
Target: 5'- --gGUCAGGGUGAGGUCGGcgUCuuGgAg -3' miRNA: 3'- cugCAGUCCCGCUUCGGCU--AGggCgU- -5' |
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18454 | 5' | -59 | NC_004681.1 | + | 990 | 0.67 | 0.530479 |
Target: 5'- gGACGcCAGGGCG--GCU--UCCCGCu -3' miRNA: 3'- -CUGCaGUCCCGCuuCGGcuAGGGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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