Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18455 | 3' | -58.6 | NC_004681.1 | + | 44291 | 0.66 | 0.619271 |
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Target: 5'- cCAUGUG-GACUGCGCaUCGGGuGUg -3' miRNA: 3'- aGUACACaCUGACGUGgGGCCCcCAg -5' |
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| 18455 | 3' | -58.6 | NC_004681.1 | + | 19941 | 0.67 | 0.55632 |
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Target: 5'- cUcgGUGUagGACaGCACCUCGaagccGGGGUCa -3' miRNA: 3'- aGuaCACA--CUGaCGUGGGGC-----CCCCAG- -5' |
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| 18455 | 3' | -58.6 | NC_004681.1 | + | 60871 | 0.67 | 0.525499 |
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Target: 5'- cUUAUGcGUGGCUGgucaagcuaguCACCCCaccuGGGGGUg -3' miRNA: 3'- -AGUACaCACUGAC-----------GUGGGG----CCCCCAg -5' |
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| 18455 | 3' | -58.6 | NC_004681.1 | + | 44371 | 0.69 | 0.428336 |
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Target: 5'- aCAUG-GUGAacucuucaUGCACgCCGGGGG-Cg -3' miRNA: 3'- aGUACaCACUg-------ACGUGgGGCCCCCaG- -5' |
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| 18455 | 3' | -58.6 | NC_004681.1 | + | 18459 | 0.69 | 0.419203 |
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Target: 5'- cCAUGUc-GAUgGCGCCaCCGGGGGUg -3' miRNA: 3'- aGUACAcaCUGaCGUGG-GGCCCCCAg -5' |
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| 18455 | 3' | -58.6 | NC_004681.1 | + | 40097 | 1.1 | 0.000547 |
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Target: 5'- aUCAUGUGUGACUGCACCCCGGGGGUCg -3' miRNA: 3'- -AGUACACACUGACGUGGGGCCCCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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