Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18456 | 5' | -57.5 | NC_004681.1 | + | 4503 | 0.66 | 0.702922 |
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Target: 5'- -aUGUGCUcgacuuCGACGUGGUCUacgacgacaUCGAGGc -3' miRNA: 3'- uaGCACGG------GCUGCAUCAGG---------AGCUCCa -5' |
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| 18456 | 5' | -57.5 | NC_004681.1 | + | 17479 | 0.67 | 0.629032 |
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Target: 5'- uUCGUGCCUGGCaUGGgcggCCUCGgagacuuccGGGUu -3' miRNA: 3'- uAGCACGGGCUGcAUCa---GGAGC---------UCCA- -5' |
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| 18456 | 5' | -57.5 | NC_004681.1 | + | 64395 | 0.68 | 0.586656 |
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Target: 5'- uUCGUGgUUGuCGUAGccaUCCUUGAGGUa -3' miRNA: 3'- uAGCACgGGCuGCAUC---AGGAGCUCCA- -5' |
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| 18456 | 5' | -57.5 | NC_004681.1 | + | 65125 | 0.7 | 0.464612 |
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Target: 5'- --aGUGCCU-ACGgcGUCCUUGGGGUu -3' miRNA: 3'- uagCACGGGcUGCauCAGGAGCUCCA- -5' |
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| 18456 | 5' | -57.5 | NC_004681.1 | + | 23681 | 0.7 | 0.464612 |
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Target: 5'- -cCGUGCCCu-CGUAGcCCUCGuGGc -3' miRNA: 3'- uaGCACGGGcuGCAUCaGGAGCuCCa -5' |
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| 18456 | 5' | -57.5 | NC_004681.1 | + | 40506 | 1.05 | 0.001883 |
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Target: 5'- aAUCGUGCCCGACGUAGUCCUCGAGGUu -3' miRNA: 3'- -UAGCACGGGCUGCAUCAGGAGCUCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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