miRNA: 3'- gCCGCuua--GGUGu--C-CGAGGU-CGUCUG- -5'
miRNA: 3'- gCCGCUu----AGGUG-UCCGagGUCGUCUG- -5'
miRNA: 3'- -GC-CGCU----UAGGUG------UCCGAGGuCGUCUG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18457 | 5' | -56.9 | NC_004681.1 | + | 65029 | 0.67 | 0.651883 |
|
Target: 5'- aGGCGcgucgCCACcucGuGCUCCAgGCGGGCg -3' miRNA: 3'- gCCGCuua--GGUGu--C-CGAGGU-CGUCUG- -5' |
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| 18457 | 5' | -56.9 | NC_004681.1 | + | 65345 | 0.66 | 0.694548 |
|
Target: 5'- aGGCGAuggcuUUCACgAGGUg-CGGCAGGCc -3' miRNA: 3'- gCCGCUu----AGGUG-UCCGagGUCGUCUG- -5' |
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| 18457 | 5' | -56.9 | NC_004681.1 | + | 75046 | 0.66 | 0.705096 |
|
Target: 5'- cCGuGCGAguagGUCCAUcagugaGGGCUCC-GUGGGCu -3' miRNA: 3'- -GC-CGCU----UAGGUG------UCCGAGGuCGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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