Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18458 | 5' | -52.5 | NC_004681.1 | + | 32563 | 0.66 | 0.863593 |
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Target: 5'- ---gCCCUgaagGGgGCCGAGGAGAA-GUGa -3' miRNA: 3'- aauaGGGG----UCgCGGCUCCUUUUaCAU- -5' |
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| 18458 | 5' | -52.5 | NC_004681.1 | + | 17933 | 0.67 | 0.836671 |
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Target: 5'- -gGUCCCCgcaggugAGUGCCGAGGGc------ -3' miRNA: 3'- aaUAGGGG-------UCGCGGCUCCUuuuacau -5' |
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| 18458 | 5' | -52.5 | NC_004681.1 | + | 59951 | 0.68 | 0.779741 |
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Target: 5'- aUGUCCCggCAGuCGCCGAGGuggaucaagacAGGAUGUu -3' miRNA: 3'- aAUAGGG--GUC-GCGGCUCC-----------UUUUACAu -5' |
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| 18458 | 5' | -52.5 | NC_004681.1 | + | 40865 | 0.69 | 0.72706 |
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Target: 5'- --uUCCUCGGCGCUGGGGAu------ -3' miRNA: 3'- aauAGGGGUCGCGGCUCCUuuuacau -5' |
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| 18458 | 5' | -52.5 | NC_004681.1 | + | 16795 | 0.71 | 0.615758 |
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Target: 5'- -cAUCCCCGGCaugcCCGAGGAccccucGGUGUAc -3' miRNA: 3'- aaUAGGGGUCGc---GGCUCCUu-----UUACAU- -5' |
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| 18458 | 5' | -52.5 | NC_004681.1 | + | 40770 | 1.04 | 0.004532 |
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Target: 5'- cUUAUCCCCAGCGCCGAGGAAAAUGUAc -3' miRNA: 3'- -AAUAGGGGUCGCGGCUCCUUUUACAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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