Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18459 | 3' | -63.6 | NC_004681.1 | + | 67801 | 0.76 | 0.089694 |
Target: 5'- aGCGccaGCGGCCCUUgucgUCGGUGCCGaCGu -3' miRNA: 3'- aCGC---UGCCGGGAGa---AGCCGCGGCgGC- -5' |
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18459 | 3' | -63.6 | NC_004681.1 | + | 68555 | 0.66 | 0.397378 |
Target: 5'- -aCGACGaggucaGCCUUCUUCGGCaGCCucaguagaguGCCGu -3' miRNA: 3'- acGCUGC------CGGGAGAAGCCG-CGG----------CGGC- -5' |
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18459 | 3' | -63.6 | NC_004681.1 | + | 75422 | 0.66 | 0.389008 |
Target: 5'- cGCG-CGGCCgUaCUcgCGGCGCaGCCc -3' miRNA: 3'- aCGCuGCCGGgA-GAa-GCCGCGgCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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