Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
18461 | 3' | -58.7 | NC_004681.1 | + | 8807 | 0.66 | 0.593377 |
Target: 5'- aGCGACCGCGGCGGcguUgUUgUGCGc -3' miRNA: 3'- aUGCUGGCGCUGCCcuuGgAGgACGU- -5' |
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18461 | 3' | -58.7 | NC_004681.1 | + | 7463 | 0.66 | 0.614425 |
Target: 5'- -uCGGCCGCGggcuugGCGGGAGgCUUCgccGCGg -3' miRNA: 3'- auGCUGGCGC------UGCCCUUgGAGGa--CGU- -5' |
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18461 | 3' | -58.7 | NC_004681.1 | + | 16398 | 0.66 | 0.635529 |
Target: 5'- -cCGGCCGCGGggaugcccuCGGGGAgCUgguagcCCUGCGc -3' miRNA: 3'- auGCUGGCGCU---------GCCCUUgGA------GGACGU- -5' |
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18461 | 3' | -58.7 | NC_004681.1 | + | 22738 | 0.66 | 0.614425 |
Target: 5'- cACGGCCGCcacuCGGGugAACCUCgaGUg -3' miRNA: 3'- aUGCUGGCGcu--GCCC--UUGGAGgaCGu -5' |
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18461 | 3' | -58.7 | NC_004681.1 | + | 22409 | 0.66 | 0.593377 |
Target: 5'- -cCGGCCGCgGACGGcAACCcUCC-GCAc -3' miRNA: 3'- auGCUGGCG-CUGCCcUUGG-AGGaCGU- -5' |
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18461 | 3' | -58.7 | NC_004681.1 | + | 8910 | 0.66 | 0.597579 |
Target: 5'- -cUGGCUgGCGGCGGGuaccccggcgucaacGGCCUgCUGCAg -3' miRNA: 3'- auGCUGG-CGCUGCCC---------------UUGGAgGACGU- -5' |
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18461 | 3' | -58.7 | NC_004681.1 | + | 46716 | 0.66 | 0.635529 |
Target: 5'- gGCGugUGCGggGCGGGGuCCUCg-GCGa -3' miRNA: 3'- aUGCugGCGC--UGCCCUuGGAGgaCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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