Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18461 | 3' | -58.7 | NC_004681.1 | + | 45161 | 0.69 | 0.415432 |
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Target: 5'- -uCGACCGCGGCGG-AugCUUCgGCGu -3' miRNA: 3'- auGCUGGCGCUGCCcUugGAGGaCGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 61850 | 0.72 | 0.301815 |
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Target: 5'- gGCGGCCGCGgucugguggcgcGCGaGGGcgGCCUCCUuGCGg -3' miRNA: 3'- aUGCUGGCGC------------UGC-CCU--UGGAGGA-CGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 18395 | 0.72 | 0.280817 |
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Target: 5'- gGCGGCCGCGGCGGcaaacGCCUUC-GCAc -3' miRNA: 3'- aUGCUGGCGCUGCCcu---UGGAGGaCGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 33753 | 0.73 | 0.254646 |
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Target: 5'- aGCGGCCgagcGCGGCuGGGAGCUUCC-GCAc -3' miRNA: 3'- aUGCUGG----CGCUG-CCCUUGGAGGaCGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 19024 | 0.73 | 0.242329 |
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Target: 5'- cUGCGcucCCGCGACGGGAGCCUggggaCCgGCc -3' miRNA: 3'- -AUGCu--GGCGCUGCCCUUGGA-----GGaCGu -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 46293 | 0.74 | 0.208335 |
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Target: 5'- cUACGuCCaCGGCGGGcAGCCaUCCUGCAu -3' miRNA: 3'- -AUGCuGGcGCUGCCC-UUGG-AGGACGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 41749 | 1.06 | 0.001123 |
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Target: 5'- cUACGACCGCGACGGGAACCUCCUGCAc -3' miRNA: 3'- -AUGCUGGCGCUGCCCUUGGAGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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