Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18461 | 3' | -58.7 | NC_004681.1 | + | 46293 | 0.74 | 0.208335 |
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Target: 5'- cUACGuCCaCGGCGGGcAGCCaUCCUGCAu -3' miRNA: 3'- -AUGCuGGcGCUGCCC-UUGG-AGGACGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 46716 | 0.66 | 0.635529 |
|
Target: 5'- gGCGugUGCGggGCGGGGuCCUCg-GCGa -3' miRNA: 3'- aUGCugGCGC--UGCCCUuGGAGgaCGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 51987 | 0.69 | 0.442039 |
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Target: 5'- cUGCGGCCaugaucuGCGagaGCGGGGACCaggugCCUGCc -3' miRNA: 3'- -AUGCUGG-------CGC---UGCCCUUGGa----GGACGu -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 52267 | 0.67 | 0.56205 |
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Target: 5'- --gGGCCGCGACGcGGAcguuguccuCCUCCUcCAg -3' miRNA: 3'- augCUGGCGCUGC-CCUu--------GGAGGAcGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 58514 | 0.68 | 0.481236 |
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Target: 5'- -uCGGCCGCGgagaagguGCGGGA-CUUCUUGCc -3' miRNA: 3'- auGCUGGCGC--------UGCCCUuGGAGGACGu -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 61773 | 0.68 | 0.510956 |
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Target: 5'- --aGACCuCGGCGGcGGCCUCCgcgGCGg -3' miRNA: 3'- augCUGGcGCUGCCcUUGGAGGa--CGU- -5' |
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| 18461 | 3' | -58.7 | NC_004681.1 | + | 61850 | 0.72 | 0.301815 |
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Target: 5'- gGCGGCCGCGgucugguggcgcGCGaGGGcgGCCUCCUuGCGg -3' miRNA: 3'- aUGCUGGCGC------------UGC-CCU--UGGAGGA-CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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