Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18462 | 3' | -57.4 | NC_004681.1 | + | 22190 | 0.69 | 0.471088 |
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Target: 5'- gAugCGGCGCUGcaACUUGGCCacaggggauuccUGCGCc -3' miRNA: 3'- aUugGCCGCGAC--UGGACUGG------------AUGCGc -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 46541 | 0.69 | 0.471088 |
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Target: 5'- -uACCGGaGCaGGCCgcggGaACCUGCGCGg -3' miRNA: 3'- auUGGCCgCGaCUGGa---C-UGGAUGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 23651 | 0.69 | 0.467186 |
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Target: 5'- gAGCCGGgugagcccaucgaGCUGGCCU--CCUACGCGc -3' miRNA: 3'- aUUGGCCg------------CGACUGGAcuGGAUGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 14319 | 0.7 | 0.461365 |
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Target: 5'- -cGCCgaGGCGCgGGCCaaUGAgCUGCGCGc -3' miRNA: 3'- auUGG--CCGCGaCUGG--ACUgGAUGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 7336 | 0.7 | 0.451748 |
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Target: 5'- --cCCGGgGCUGACCccGCCgcCGCGg -3' miRNA: 3'- auuGGCCgCGACUGGacUGGauGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 17905 | 0.71 | 0.396485 |
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Target: 5'- cGGCCGccGCGUUGAUgUcGGCCUGCGUGg -3' miRNA: 3'- aUUGGC--CGCGACUGgA-CUGGAUGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 29806 | 0.71 | 0.387706 |
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Target: 5'- cAGCUGcGCGC-GGCCggucGACCUACGCc -3' miRNA: 3'- aUUGGC-CGCGaCUGGa---CUGGAUGCGc -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 30158 | 0.72 | 0.345769 |
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Target: 5'- cAGgCGGCGC-GGCCgcggagugGACCUACGCc -3' miRNA: 3'- aUUgGCCGCGaCUGGa-------CUGGAUGCGc -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 62350 | 0.72 | 0.314631 |
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Target: 5'- --cCCGGCGCggugGACgaaGACCUugGCGa -3' miRNA: 3'- auuGGCCGCGa---CUGga-CUGGAugCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 41971 | 1.08 | 0.001075 |
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Target: 5'- uUAACCGGCGCUGACCUGACCUACGCGg -3' miRNA: 3'- -AUUGGCCGCGACUGGACUGGAUGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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