Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18462 | 3' | -57.4 | NC_004681.1 | + | 40071 | 0.67 | 0.626294 |
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Target: 5'- gAGCCGGUgaGUUGACCcgGcGCCgACGCGc -3' miRNA: 3'- aUUGGCCG--CGACUGGa-C-UGGaUGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 40168 | 0.68 | 0.573165 |
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Target: 5'- cAGCCugucgaucugGGUGCUGGCgUcGGCCUGCGgGa -3' miRNA: 3'- aUUGG----------CCGCGACUGgA-CUGGAUGCgC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 41971 | 1.08 | 0.001075 |
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Target: 5'- uUAACCGGCGCUGACCUGACCUACGCGg -3' miRNA: 3'- -AUUGGCCGCGACUGGACUGGAUGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 42052 | 0.66 | 0.668929 |
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Target: 5'- aGugCaGCGCUccagucagccaACCUGGCCUACGCc -3' miRNA: 3'- aUugGcCGCGAc----------UGGACUGGAUGCGc -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 46541 | 0.69 | 0.471088 |
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Target: 5'- -uACCGGaGCaGGCCgcggGaACCUGCGCGg -3' miRNA: 3'- auUGGCCgCGaCUGGa---C-UGGAUGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 49352 | 0.69 | 0.500857 |
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Target: 5'- -cGCgGGCGCUGACCacugGGCgcacaccuuCUGCGUGa -3' miRNA: 3'- auUGgCCGCGACUGGa---CUG---------GAUGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 50252 | 0.67 | 0.604969 |
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Target: 5'- gGAUCaaGGCGCUGuCCUGGuCCcGCGCc -3' miRNA: 3'- aUUGG--CCGCGACuGGACU-GGaUGCGc -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 55803 | 0.66 | 0.668929 |
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Target: 5'- aGAgCGGCuGCUGACggucgaUGACCUcaagGCGCu -3' miRNA: 3'- aUUgGCCG-CGACUGg-----ACUGGA----UGCGc -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 60053 | 0.67 | 0.604969 |
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Target: 5'- -cACCuGGCaccgGCUGACCgUGGCCaucgaggACGCGg -3' miRNA: 3'- auUGG-CCG----CGACUGG-ACUGGa------UGCGC- -5' |
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| 18462 | 3' | -57.4 | NC_004681.1 | + | 62350 | 0.72 | 0.314631 |
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Target: 5'- --cCCGGCGCggugGACgaaGACCUugGCGa -3' miRNA: 3'- auuGGCCGCGa---CUGga-CUGGAugCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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