Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18463 | 3' | -57.1 | NC_004681.1 | + | 7005 | 0.66 | 0.666285 |
Target: 5'- gAGCCcacGGUGGuGGCCAcggacAUGCCGUg- -3' miRNA: 3'- gUCGGa--CUAUCuCCGGU-----UGCGGCGau -5' |
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18463 | 3' | -57.1 | NC_004681.1 | + | 3238 | 0.68 | 0.537047 |
Target: 5'- aGGcCCUGGaGGAGGgCGA-GCCGCUGg -3' miRNA: 3'- gUC-GGACUaUCUCCgGUUgCGGCGAU- -5' |
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18463 | 3' | -57.1 | NC_004681.1 | + | 1478 | 0.67 | 0.558229 |
Target: 5'- aGGCCcGAUAGGuGGCCAcCGCCucGCa- -3' miRNA: 3'- gUCGGaCUAUCU-CCGGUuGCGG--CGau -5' |
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18463 | 3' | -57.1 | NC_004681.1 | + | 1398 | 0.67 | 0.612053 |
Target: 5'- gGGCCugccgcaagaUGAUGGGccacgaauacacGGCCAuugGCGCCGCg- -3' miRNA: 3'- gUCGG----------ACUAUCU------------CCGGU---UGCGGCGau -5' |
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18463 | 3' | -57.1 | NC_004681.1 | + | 1008 | 0.69 | 0.44593 |
Target: 5'- gAGCUcgagGAUGGAGGCgGACGCCaggGCg- -3' miRNA: 3'- gUCGGa---CUAUCUCCGgUUGCGG---CGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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