Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18463 | 3' | -57.1 | NC_004681.1 | + | 45097 | 0.67 | 0.557164 |
Target: 5'- nCGGUCUcgcucaaGAccGGGGCCGACGCCGUc- -3' miRNA: 3'- -GUCGGA-------CUauCUCCGGUUGCGGCGau -5' |
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18463 | 3' | -57.1 | NC_004681.1 | + | 1398 | 0.67 | 0.612053 |
Target: 5'- gGGCCugccgcaagaUGAUGGGccacgaauacacGGCCAuugGCGCCGCg- -3' miRNA: 3'- gUCGG----------ACUAUCU------------CCGGU---UGCGGCGau -5' |
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18463 | 3' | -57.1 | NC_004681.1 | + | 8282 | 0.66 | 0.622902 |
Target: 5'- gAGCCUcGGUGGc-GCgGACGCCGCc- -3' miRNA: 3'- gUCGGA-CUAUCucCGgUUGCGGCGau -5' |
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18463 | 3' | -57.1 | NC_004681.1 | + | 7577 | 0.66 | 0.666286 |
Target: 5'- aCGGCCgg--GGAGGCCGugauggcaccuACGcCCGCg- -3' miRNA: 3'- -GUCGGacuaUCUCCGGU-----------UGC-GGCGau -5' |
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18463 | 3' | -57.1 | NC_004681.1 | + | 42146 | 1.07 | 0.001131 |
Target: 5'- cCAGCCUGAUAGAGGCCAACGCCGCUAa -3' miRNA: 3'- -GUCGGACUAUCUCCGGUUGCGGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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