Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18463 | 5' | -57.3 | NC_004681.1 | + | 57922 | 0.67 | 0.640784 |
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Target: 5'- --gGAGGG-GCCAGCC-CCGcUCcuuCGCc -3' miRNA: 3'- aagCUCCCaCGGUUGGaGGC-AGu--GCG- -5' |
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| 18463 | 5' | -57.3 | NC_004681.1 | + | 41995 | 0.67 | 0.651435 |
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Target: 5'- -gCGGGGGcgaucucgGCCAGCCU-UGUCgACGCc -3' miRNA: 3'- aaGCUCCCa-------CGGUUGGAgGCAG-UGCG- -5' |
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| 18463 | 5' | -57.3 | NC_004681.1 | + | 44213 | 0.66 | 0.66207 |
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Target: 5'- cUCGGGGGUGaggCAgguGCCUuuGUgGCGg -3' miRNA: 3'- aAGCUCCCACg--GU---UGGAggCAgUGCg -5' |
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| 18463 | 5' | -57.3 | NC_004681.1 | + | 12754 | 0.66 | 0.66207 |
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Target: 5'- -aUGGGGGUGCCcccauCCacgCCGUCA-GCc -3' miRNA: 3'- aaGCUCCCACGGuu---GGa--GGCAGUgCG- -5' |
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| 18463 | 5' | -57.3 | NC_004681.1 | + | 18819 | 0.66 | 0.682196 |
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Target: 5'- -cCGAGGGUgaaGUCAGCCUCaugggggCGgagCugGCg -3' miRNA: 3'- aaGCUCCCA---CGGUUGGAG-------GCa--GugCG- -5' |
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| 18463 | 5' | -57.3 | NC_004681.1 | + | 48674 | 0.66 | 0.692728 |
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Target: 5'- -gCGAGGGcgugGCCcacgaugcagcagGGCUgggugcugCCGUCGCGCu -3' miRNA: 3'- aaGCUCCCa---CGG-------------UUGGa-------GGCAGUGCG- -5' |
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| 18463 | 5' | -57.3 | NC_004681.1 | + | 48118 | 0.66 | 0.704249 |
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Target: 5'- -gCGGGGG-GCuCAACCaUCUG-CACGUc -3' miRNA: 3'- aaGCUCCCaCG-GUUGG-AGGCaGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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