Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18464 | 5' | -57.1 | NC_004681.1 | + | 45020 | 0.7 | 0.476177 |
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Target: 5'- cACCGCGUgGGgGCGAgcucUUugGCCUc -3' miRNA: 3'- -UGGUGCAgCCgCGCUaac-AGugCGGA- -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 61320 | 0.7 | 0.476177 |
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Target: 5'- cGCCACGuucUCGGCG-Gc--GUCGCGCCa -3' miRNA: 3'- -UGGUGC---AGCCGCgCuaaCAGUGCGGa -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 50452 | 0.7 | 0.467422 |
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Target: 5'- uACCACGUCGuGgGCGAcgcccaccccgaccgUGUCcuGCGCCa -3' miRNA: 3'- -UGGUGCAGC-CgCGCUa--------------ACAG--UGCGGa -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 70378 | 0.7 | 0.456836 |
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Target: 5'- aACCGCG-CGGUa-GAUUGUCACGCg- -3' miRNA: 3'- -UGGUGCaGCCGcgCUAACAGUGCGga -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 8804 | 0.7 | 0.456836 |
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Target: 5'- gACCGCGgCGGCGUuGUUGUgCGCaGCCg -3' miRNA: 3'- -UGGUGCaGCCGCGcUAACA-GUG-CGGa -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 24057 | 0.71 | 0.437925 |
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Target: 5'- uACCAgGUCGGCGaacguGUUGgCACGCCc -3' miRNA: 3'- -UGGUgCAGCCGCgc---UAACaGUGCGGa -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 33374 | 0.73 | 0.334696 |
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Target: 5'- gGCCGCGUCGcGCGCGccgUGUgGCaCCUg -3' miRNA: 3'- -UGGUGCAGC-CGCGCua-ACAgUGcGGA- -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 34346 | 0.75 | 0.23821 |
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Target: 5'- cGCCGCGgCGGCGUGAgggUUGUgCGCGCUg -3' miRNA: 3'- -UGGUGCaGCCGCGCU---AACA-GUGCGGa -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 53042 | 0.76 | 0.226498 |
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Target: 5'- gGCCACGUCGGUGaCGAUgaagcgCACGUCg -3' miRNA: 3'- -UGGUGCAGCCGC-GCUAaca---GUGCGGa -5' |
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| 18464 | 5' | -57.1 | NC_004681.1 | + | 42463 | 1.06 | 0.001599 |
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Target: 5'- uACCACGUCGGCGCGAUUGUCACGCCUc -3' miRNA: 3'- -UGGUGCAGCCGCGCUAACAGUGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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