Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18468 | 3' | -56.6 | NC_004681.1 | + | 52550 | 0.66 | 0.743322 |
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Target: 5'- cCUUCGacGCuaucgcgGACgaguGGUCCCCUCCg -3' miRNA: 3'- -GAAGC--CGuua----CUGac--CCAGGGGAGGa -5' |
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| 18468 | 3' | -56.6 | NC_004681.1 | + | 44563 | 0.66 | 0.726929 |
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Target: 5'- --gUGGCGAcUGACUGGGUagaaaggaggaaagaCCCCagCCa -3' miRNA: 3'- gaaGCCGUU-ACUGACCCA---------------GGGGa-GGa -5' |
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| 18468 | 3' | -56.6 | NC_004681.1 | + | 56117 | 0.66 | 0.712396 |
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Target: 5'- ---aGGCGAUG-CgGaGGUCgCCCUCCUu -3' miRNA: 3'- gaagCCGUUACuGaC-CCAG-GGGAGGA- -5' |
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| 18468 | 3' | -56.6 | NC_004681.1 | + | 11526 | 0.66 | 0.701924 |
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Target: 5'- --aCGGCccgcuACUGGGUCCaCCUUCg -3' miRNA: 3'- gaaGCCGuuac-UGACCCAGG-GGAGGa -5' |
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| 18468 | 3' | -56.6 | NC_004681.1 | + | 10377 | 0.67 | 0.680797 |
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Target: 5'- gCUUCGGCAGUGucGCUGGcaCCUCgcugUCCa -3' miRNA: 3'- -GAAGCCGUUAC--UGACCcaGGGG----AGGa -5' |
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| 18468 | 3' | -56.6 | NC_004681.1 | + | 53188 | 0.67 | 0.633809 |
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Target: 5'- ---aGGCAGUGACgucgauguagcgcGGGUCCCgaUCCg -3' miRNA: 3'- gaagCCGUUACUGa------------CCCAGGGg-AGGa -5' |
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| 18468 | 3' | -56.6 | NC_004681.1 | + | 33951 | 0.68 | 0.616674 |
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Target: 5'- --gUGGauGUGACUGaGGUCCCgUCCg -3' miRNA: 3'- gaaGCCguUACUGAC-CCAGGGgAGGa -5' |
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| 18468 | 3' | -56.6 | NC_004681.1 | + | 12763 | 0.71 | 0.423864 |
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Target: 5'- --gCGGCGGUGAUggGGGUgCCCCcaUCCa -3' miRNA: 3'- gaaGCCGUUACUGa-CCCA-GGGG--AGGa -5' |
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| 18468 | 3' | -56.6 | NC_004681.1 | + | 45017 | 1.08 | 0.001253 |
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Target: 5'- gCUUCGGCAAUGACUGGGUCCCCUCCUa -3' miRNA: 3'- -GAAGCCGUUACUGACCCAGGGGAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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