Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18473 | 3' | -60.4 | NC_004681.1 | + | 10299 | 0.66 | 0.529777 |
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Target: 5'- -cAGGUGuGGCCGguGCUCCACucggagccGGGGAg -3' miRNA: 3'- acUUCAC-CCGGC--UGAGGUGc-------CCCCUa -5' |
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| 18473 | 3' | -60.4 | NC_004681.1 | + | 39348 | 0.66 | 0.49979 |
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Target: 5'- -cGAG-GGGCCG-CUguggaucggCCugGGGGGAa -3' miRNA: 3'- acUUCaCCCGGCuGA---------GGugCCCCCUa -5' |
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| 18473 | 3' | -60.4 | NC_004681.1 | + | 30069 | 0.67 | 0.442262 |
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Target: 5'- gGggGuUGGGCCGACcUgGCGGGcaaGGGUu -3' miRNA: 3'- aCuuC-ACCCGGCUGaGgUGCCC---CCUA- -5' |
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| 18473 | 3' | -60.4 | NC_004681.1 | + | 49651 | 0.7 | 0.309333 |
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Target: 5'- -----cGGGCCuGC-CCGCGGGGGAa -3' miRNA: 3'- acuucaCCCGGcUGaGGUGCCCCCUa -5' |
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| 18473 | 3' | -60.4 | NC_004681.1 | + | 8631 | 0.72 | 0.219916 |
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Target: 5'- aGggGUucuGGCCGAUggCCGCGGGGGc- -3' miRNA: 3'- aCuuCAc--CCGGCUGa-GGUGCCCCCua -5' |
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| 18473 | 3' | -60.4 | NC_004681.1 | + | 46555 | 1.06 | 0.000764 |
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Target: 5'- gUGAAGUGGGCCGACUCCACGGGGGAUg -3' miRNA: 3'- -ACUUCACCCGGCUGAGGUGCCCCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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