Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18477 | 5' | -51.6 | NC_004681.1 | + | 17367 | 0.67 | 0.919992 |
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Target: 5'- ---cGCCGAcUGCUccagCGCGGUCGAGg- -3' miRNA: 3'- gugaCGGUU-AUGAa---GUGCCAGCUCgc -5' |
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| 18477 | 5' | -51.6 | NC_004681.1 | + | 16124 | 0.67 | 0.913784 |
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Target: 5'- gCugUGUCGGguaucgGCUUCGCGG-CGcuGGCGg -3' miRNA: 3'- -GugACGGUUa-----UGAAGUGCCaGC--UCGC- -5' |
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| 18477 | 5' | -51.6 | NC_004681.1 | + | 2980 | 0.67 | 0.893513 |
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Target: 5'- ---gGUCGAgcgggagggggGCUucgUCACGGUCGAGCGc -3' miRNA: 3'- gugaCGGUUa----------UGA---AGUGCCAGCUCGC- -5' |
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| 18477 | 5' | -51.6 | NC_004681.1 | + | 4943 | 0.68 | 0.886958 |
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Target: 5'- uCACUGCgguggguacggcguCGAUGCgcuuggcagccaugUCGCGGUCGGGUu -3' miRNA: 3'- -GUGACG--------------GUUAUGa-------------AGUGCCAGCUCGc -5' |
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| 18477 | 5' | -51.6 | NC_004681.1 | + | 6096 | 0.69 | 0.809606 |
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Target: 5'- ---cGCCGcagaccuCUUCGCGGUUGAGUGg -3' miRNA: 3'- gugaCGGUuau----GAAGUGCCAGCUCGC- -5' |
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| 18477 | 5' | -51.6 | NC_004681.1 | + | 6782 | 0.74 | 0.553669 |
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Target: 5'- cCGCUcgGCCAGUGCgagcugCACGG-CGAGCa -3' miRNA: 3'- -GUGA--CGGUUAUGaa----GUGCCaGCUCGc -5' |
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| 18477 | 5' | -51.6 | NC_004681.1 | + | 48528 | 1.08 | 0.003955 |
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Target: 5'- gCACUGCCAAUACUUCACGGUCGAGCGc -3' miRNA: 3'- -GUGACGGUUAUGAAGUGCCAGCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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