Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18478 | 3' | -58.1 | NC_004681.1 | + | 63944 | 0.67 | 0.561804 |
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Target: 5'- cGAGGCaAGGC--CCGCCcGGAuuugaaccggGGACGa -3' miRNA: 3'- uCUCCG-UCCGuaGGCGGuUCU----------CCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 50410 | 0.67 | 0.572283 |
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Target: 5'- cGGGGguGcccaugaguucaGCGUCgGCCGcGGGGACGu -3' miRNA: 3'- uCUCCguC------------CGUAGgCGGUuCUCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 63211 | 0.67 | 0.572283 |
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Target: 5'- aAGAGGUucGGC-UUCGCCcGGuGGGACGu -3' miRNA: 3'- -UCUCCGu-CCGuAGGCGGuUC-UCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 64610 | 0.67 | 0.593372 |
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Target: 5'- uGGGGGCAgGGUGUUCGUCAGGugaccGGCGa -3' miRNA: 3'- -UCUCCGU-CCGUAGGCGGUUCuc---CUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 51609 | 0.66 | 0.625214 |
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Target: 5'- cGGAGGUgguGGCGUCgguguaGCCGAGcuGGCGg -3' miRNA: 3'- -UCUCCGu--CCGUAGg-----CGGUUCucCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 17273 | 0.66 | 0.635851 |
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Target: 5'- cGGGcGGCGGGUAUCC-CgGAGAcGACGc -3' miRNA: 3'- -UCU-CCGUCCGUAGGcGgUUCUcCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 36231 | 0.66 | 0.646484 |
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Target: 5'- gAGAGuGCGGGCAgaggcuaugaacUCgGCCAcugGGAGGuCu -3' miRNA: 3'- -UCUC-CGUCCGU------------AGgCGGU---UCUCCuGc -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 8288 | 0.66 | 0.657105 |
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Target: 5'- cGGuGGCGcGGacgCCGCCA--AGGACGg -3' miRNA: 3'- -UCuCCGU-CCguaGGCGGUucUCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 65006 | 0.66 | 0.657105 |
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Target: 5'- -cAGGCGGGCG-CCGC-GGGAGaGAUGc -3' miRNA: 3'- ucUCCGUCCGUaGGCGgUUCUC-CUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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