Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18478 | 3' | -58.1 | NC_004681.1 | + | 2972 | 0.69 | 0.441885 |
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Target: 5'- gGGAGGgGGGCuUCgucacggucgagCGCCucGGGGACGg -3' miRNA: 3'- -UCUCCgUCCGuAG------------GCGGuuCUCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 49971 | 0.7 | 0.395425 |
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Target: 5'- uAGAGcaguggcguuucuGCGGGguUCUGCCAGGGgcGGACGu -3' miRNA: 3'- -UCUC-------------CGUCCguAGGCGGUUCU--CCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 14560 | 0.71 | 0.353053 |
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Target: 5'- cGGAGGCGGcGCGcgcggcguucgccUCCGCCucGcGGGCGg -3' miRNA: 3'- -UCUCCGUC-CGU-------------AGGCGGuuCuCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 14451 | 0.72 | 0.310274 |
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Target: 5'- cGAGGCGGaggcgaacgccgcgcGCGccgccUCCGCCGAGcAGGGCGc -3' miRNA: 3'- uCUCCGUC---------------CGU-----AGGCGGUUC-UCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 29547 | 0.72 | 0.307321 |
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Target: 5'- --cGGCucGGGCAUcgCCGCCAAGuGGGCa -3' miRNA: 3'- ucuCCG--UCCGUA--GGCGGUUCuCCUGc -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 24217 | 0.74 | 0.246522 |
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Target: 5'- uGAGGaacacguugaAGGCGUCCGCCAGGcuGGCGc -3' miRNA: 3'- uCUCCg---------UCCGUAGGCGGUUCucCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 72033 | 0.75 | 0.201165 |
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Target: 5'- uGAGGCAuacGGCaAUCCgGCCGAuGAGGACa -3' miRNA: 3'- uCUCCGU---CCG-UAGG-CGGUU-CUCCUGc -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 26323 | 0.78 | 0.13187 |
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Target: 5'- aGGGGGCGGGCAUcgCCGCCGuggcgGGccAGGACGu -3' miRNA: 3'- -UCUCCGUCCGUA--GGCGGU-----UC--UCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 48599 | 1.09 | 0.000712 |
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Target: 5'- cAGAGGCAGGCAUCCGCCAAGAGGACGg -3' miRNA: 3'- -UCUCCGUCCGUAGGCGGUUCUCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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