Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18478 | 3' | -58.1 | NC_004681.1 | + | 10005 | 0.69 | 0.4804 |
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Target: 5'- cGGGGaAGGCGUCgGCCAGcugcuGGGCGa -3' miRNA: 3'- uCUCCgUCCGUAGgCGGUUcu---CCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 41715 | 0.69 | 0.451352 |
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Target: 5'- uGGAGGUGGGCGuUUCGUCGuggcGGAGGAg- -3' miRNA: 3'- -UCUCCGUCCGU-AGGCGGU----UCUCCUgc -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 2972 | 0.69 | 0.441885 |
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Target: 5'- gGGAGGgGGGCuUCgucacggucgagCGCCucGGGGACGg -3' miRNA: 3'- -UCUCCgUCCGuAG------------GCGGuuCUCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 49971 | 0.7 | 0.395425 |
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Target: 5'- uAGAGcaguggcguuucuGCGGGguUCUGCCAGGGgcGGACGu -3' miRNA: 3'- -UCUC-------------CGUCCguAGGCGGUUCU--CCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 14560 | 0.71 | 0.353053 |
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Target: 5'- cGGAGGCGGcGCGcgcggcguucgccUCCGCCucGcGGGCGg -3' miRNA: 3'- -UCUCCGUC-CGU-------------AGGCGGuuCuCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 24217 | 0.74 | 0.246522 |
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Target: 5'- uGAGGaacacguugaAGGCGUCCGCCAGGcuGGCGc -3' miRNA: 3'- uCUCCg---------UCCGUAGGCGGUUCucCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 72033 | 0.75 | 0.201165 |
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Target: 5'- uGAGGCAuacGGCaAUCCgGCCGAuGAGGACa -3' miRNA: 3'- uCUCCGU---CCG-UAGG-CGGUU-CUCCUGc -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 26323 | 0.78 | 0.13187 |
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Target: 5'- aGGGGGCGGGCAUcgCCGCCGuggcgGGccAGGACGu -3' miRNA: 3'- -UCUCCGUCCGUA--GGCGGU-----UC--UCCUGC- -5' |
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| 18478 | 3' | -58.1 | NC_004681.1 | + | 65006 | 0.66 | 0.657105 |
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Target: 5'- -cAGGCGGGCG-CCGC-GGGAGaGAUGc -3' miRNA: 3'- ucUCCGUCCGUaGGCGgUUCUC-CUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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