Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18480 | 5' | -59.2 | NC_004681.1 | + | 62952 | 0.67 | 0.552022 |
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Target: 5'- uCCUUCgGCgguggUGGCGGCGGCGGCGc--- -3' miRNA: 3'- cGGGAG-CG-----ACUGCUGCCGCCGUagag -5' |
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| 18480 | 5' | -59.2 | NC_004681.1 | + | 51544 | 0.67 | 0.562292 |
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Target: 5'- uGCCUggcuUUGUccuUGACGAUGGUGGCGUacgUCg -3' miRNA: 3'- -CGGG----AGCG---ACUGCUGCCGCCGUAg--AG- -5' |
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| 18480 | 5' | -59.2 | NC_004681.1 | + | 14572 | 0.66 | 0.572614 |
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Target: 5'- cGCCCUgcucggCGgaGGCGGCGcgcGCGGCGUucgcCUCc -3' miRNA: 3'- -CGGGA------GCgaCUGCUGC---CGCCGUA----GAG- -5' |
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| 18480 | 5' | -59.2 | NC_004681.1 | + | 69213 | 0.66 | 0.58298 |
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Target: 5'- gGCCagggCGCgGGCGA-GGCGGCGcUUCa -3' miRNA: 3'- -CGGga--GCGaCUGCUgCCGCCGUaGAG- -5' |
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| 18480 | 5' | -59.2 | NC_004681.1 | + | 23669 | 0.66 | 0.587137 |
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Target: 5'- aGCCCUCgugGCUGACGAacacugugccagagaCGGUGGUg---- -3' miRNA: 3'- -CGGGAG---CGACUGCU---------------GCCGCCGuagag -5' |
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| 18480 | 5' | -59.2 | NC_004681.1 | + | 41964 | 0.66 | 0.603815 |
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Target: 5'- cGCCUgcuuaaccggCGCUGACcugaccuacGCGGgGGCgAUCUCg -3' miRNA: 3'- -CGGGa---------GCGACUGc--------UGCCgCCG-UAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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