Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18480 | 5' | -59.2 | NC_004681.1 | + | 47876 | 0.69 | 0.399005 |
Target: 5'- gGCgCCUCGCgGGUGAauGCGGCAUCg- -3' miRNA: 3'- -CG-GGAGCGaCUGCUgcCGCCGUAGag -5' |
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18480 | 5' | -59.2 | NC_004681.1 | + | 22589 | 0.7 | 0.348973 |
Target: 5'- cGUCCgaggCGCcGAUGAUGGUGGCGUUg- -3' miRNA: 3'- -CGGGa---GCGaCUGCUGCCGCCGUAGag -5' |
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18480 | 5' | -59.2 | NC_004681.1 | + | 39289 | 0.72 | 0.296468 |
Target: 5'- cGUCCUCGacguCGAgGGCGaGCAUCUCc -3' miRNA: 3'- -CGGGAGCgacuGCUgCCGC-CGUAGAG- -5' |
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18480 | 5' | -59.2 | NC_004681.1 | + | 45767 | 0.73 | 0.25031 |
Target: 5'- uUCCUCGCgGACaACGGUGGgCGUCUUg -3' miRNA: 3'- cGGGAGCGaCUGcUGCCGCC-GUAGAG- -5' |
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18480 | 5' | -59.2 | NC_004681.1 | + | 7989 | 0.77 | 0.125018 |
Target: 5'- uUCCUCGgaGGCGGCGGCGGU--CUCa -3' miRNA: 3'- cGGGAGCgaCUGCUGCCGCCGuaGAG- -5' |
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18480 | 5' | -59.2 | NC_004681.1 | + | 23669 | 0.66 | 0.587137 |
Target: 5'- aGCCCUCgugGCUGACGAacacugugccagagaCGGUGGUg---- -3' miRNA: 3'- -CGGGAG---CGACUGCU---------------GCCGCCGuagag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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