Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18483 | 3' | -52.9 | NC_004681.1 | + | 39282 | 0.68 | 0.829354 |
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Target: 5'- --gACGUCgaggGCGAGCaucUCCgCGGCCCg -3' miRNA: 3'- cgaUGUAGa---UGCUUG---AGGgGCUGGGg -5' |
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| 18483 | 3' | -52.9 | NC_004681.1 | + | 62391 | 0.68 | 0.820394 |
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Target: 5'- cGCUucCAUCccgcguCGAACUCCUCGgcggugaucuuGCCCCc -3' miRNA: 3'- -CGAu-GUAGau----GCUUGAGGGGC-----------UGGGG- -5' |
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| 18483 | 3' | -52.9 | NC_004681.1 | + | 7677 | 0.68 | 0.820394 |
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Target: 5'- gGUgcCAUC-ACGGcCUCCCCGGCCguCCu -3' miRNA: 3'- -CGauGUAGaUGCUuGAGGGGCUGG--GG- -5' |
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| 18483 | 3' | -52.9 | NC_004681.1 | + | 45025 | 0.69 | 0.782744 |
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Target: 5'- gGCUccACcgCguggggGCGAGCUCUuuGGCCUCu -3' miRNA: 3'- -CGA--UGuaGa-----UGCUUGAGGggCUGGGG- -5' |
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| 18483 | 3' | -52.9 | NC_004681.1 | + | 32318 | 0.7 | 0.736513 |
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Target: 5'- cGCUGCcgCUGCGAuggccuGCUCCaCCagguugccgaugaccGugCCCg -3' miRNA: 3'- -CGAUGuaGAUGCU------UGAGG-GG---------------CugGGG- -5' |
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| 18483 | 3' | -52.9 | NC_004681.1 | + | 39671 | 0.72 | 0.625704 |
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Target: 5'- ---uCGUCcgaUGCGAGCUCCUCGGCCgCg -3' miRNA: 3'- cgauGUAG---AUGCUUGAGGGGCUGGgG- -5' |
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| 18483 | 3' | -52.9 | NC_004681.1 | + | 55127 | 0.66 | 0.919157 |
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Target: 5'- cGCUACGUCa--GAAgUUCCCCGGgCgCg -3' miRNA: 3'- -CGAUGUAGaugCUU-GAGGGGCUgGgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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