Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18487 | 3' | -57.4 | NC_004681.1 | + | 48794 | 0.66 | 0.703415 |
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Target: 5'- cCGUCGAggGCgcGGGUGACaucugaugGUUGCCGUc -3' miRNA: 3'- aGUAGCU--UGa-CCCGCUGg-------CGACGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 28260 | 0.66 | 0.703415 |
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Target: 5'- gCGUCaguGCc-GGUG-CCGCUGCCGCc -3' miRNA: 3'- aGUAGcu-UGacCCGCuGGCGACGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 16248 | 0.66 | 0.703415 |
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Target: 5'- ---aUGAGCgacGGGaUGGCCGCcagcGCCGCg -3' miRNA: 3'- aguaGCUUGa--CCC-GCUGGCGa---CGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 45578 | 0.66 | 0.710654 |
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Target: 5'- cUCGUUGAuCaGGGUGGCCagcucggaguggacGCUGUCGUu -3' miRNA: 3'- -AGUAGCUuGaCCCGCUGG--------------CGACGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 62399 | 0.66 | 0.724002 |
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Target: 5'- aCAUcCGGguacuggcaugGCUGGGCGccgacACCGCccucuccGCCGCc -3' miRNA: 3'- aGUA-GCU-----------UGACCCGC-----UGGCGa------CGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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