miRNA: 3'- -AGUAGCuu----GAC-CCGCUGG--CgACGGCG- -5'
miRNA: 3'- -AGUAGCU---UGa-CCCGC---UGGCGa--CGGCG- -5'
miRNA: 3'- aGUAGCUUGa-----CCCgcuGGCGACGGCG- -5'
miRNA: 3'- aguaGCUUGACCCGCU--------GGCGACGGCg -5'
miRNA: 3'- -AGUAGCUUGACCCGCUGGCGACGGCG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18487 | 3' | -57.4 | NC_004681.1 | + | 66922 | 0.74 | 0.276624 |
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Target: 5'- gUCGUCGuugacuCUGuGGCGGCCcuGgUGCCGCg -3' miRNA: 3'- -AGUAGCuu----GAC-CCGCUGG--CgACGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 46944 | 0.75 | 0.221193 |
|
Target: 5'- aUCAUCGGgguGCcgGGGCGguuGCCGCgggGCUGCg -3' miRNA: 3'- -AGUAGCU---UGa-CCCGC---UGGCGa--CGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 60229 | 0.77 | 0.175512 |
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Target: 5'- aCGUCGAGCgacuugGGGaacCCGCUGCCGUa -3' miRNA: 3'- aGUAGCUUGa-----CCCgcuGGCGACGGCG- -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 51700 | 0.78 | 0.142158 |
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Target: 5'- ---aCGAGCUGGGCGAagccgagcCCGUUGCCGg -3' miRNA: 3'- aguaGCUUGACCCGCU--------GGCGACGGCg -5' |
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| 18487 | 3' | -57.4 | NC_004681.1 | + | 51477 | 1.11 | 0.00075 |
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Target: 5'- cUCAUCGAACUGGGCGACCGCUGCCGCc -3' miRNA: 3'- -AGUAGCUUGACCCGCUGGCGACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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